Coding

Part:BBa_J58008:Design

Designed by: Caterina Mata Garcia   Group: iGEM06_Valencia   (2006-10-23)


Periplasmic binding protein that docks a vanillin molecule


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have used the EG10815 rbsB D-ribose sequence as template to introduce 14 mutations at the level of aminoacids. With this modifications we get specificity in order to docks a vanillin molecule. We have used E.Coli most common codons for the mutations.

In addition, we check in the sequence that the following restriction sites do not appear:

1) EcoRI, SpeI, XbaI, PstI, NotI

2) ApoI, Mfe I, AvrII, NheI, NsiI, SbfI

3) AarI, BbsI, BbvCI, BsmBI, BsaI, BspMI, EarI, Nt.BstNBI

4) HindIII, BamHI, XhoI, NcoI, SacI, NdeI

Source

We have used the sequence of the coding protein rbsB D-ribose from the E. coli K12 strain.

References

L.L. Looger, M.A. Dwyer, J. Smith, and H.W. Hellinga. Computational design of receptor and sensor proteins with novel functions. Nature (2003), 423, 185-190.